› 7eld

Electron Microscopy
4.6Å resolution

Cryo-EM structure of Arabidopsis DCL1 in complex with pri-miRNA 166f

Released:
DOI: 10.2210/pdb7eld/pdb
PDB entry 7eld coloured by chain, front view.
PDB entry 7eld coloured by chain, side view.
PDB entry 7eld coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis of microRNA processing by Dicer-like 1.
Wei X, Ke H, Wen A, Gao B, Shi J, Feng Y
Nat Plants 7 1389-1396 (2021)
PMID: 34593993
Related structures: EMD-31181

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193493 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Endoribonuclease Dicer homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 1909 amino acids
Theoretical weight: 213.86 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9SP32 (Residues: 1-1909; Coverage: 100%)
Gene names: ASU1, At1g01040, CAF SIN1, DCL1, SUS1, T25K16.4
Sequence domains:
pri-miRNA 166f Chain: B
Molecule details ›
Chain: B
Length: 148 nucleotides
Theoretical weight: 47.2 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
Sequence domains: mir-166 microRNA precursor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.6Å
Relevant EMDB volumes: EMD-31181
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

11 review citations

A Structural View of miRNA Biogenesis and Function.
Leitão et al. (2022)
10 more

3 mentions without citation

Dicer structure and function: conserved and evolving features.
Zapletal et al. (2023)
2 more