› 7elh

Electron Microscopy
3.3Å resolution

In situ structure of transcriptional enzyme complex and capsid shell protein of mammalian reovirus at initiation state

Released:
DOI: 10.2210/pdb7elh/pdb
PDB entry 7elh coloured by chain, front view.
PDB entry 7elh coloured by chain, side view.
PDB entry 7elh coloured by chain, top view.
Source organism: Mammalian orthoreovirus 3
Primary publication:
Asymmetric reconstruction of mammalian reovirus reveals interactions among RNA, transcriptional factor µ2 and capsid proteins.
Pan M, Alvarez-Cabrera AL, Kang JS, Wang L, Fan C, Zhou ZH
Nat Commun 12 4176 (2021)
PMID: 34234134
Related structures: EMD-31183

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 26-mer (preferred)
PDBe Complex ID:
PDB-CPX-101000 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (6 distinct):
Mu2 Chain: A
Molecule details ›
Chain: A
Length: 736 amino acids
Theoretical weight: 83.33 KDa
Source organism: Mammalian orthoreovirus 3
UniProt:
  • Canonical: Q6EDZ8 (Residues: 1-736; Coverage: 100%)
Sequence domains: Reovirus minor core protein Mu-2
RNA-directed RNA polymerase Chain: B
Molecule details ›
Chain: B
Length: 1267 amino acids
Theoretical weight: 142.45 KDa
Source organism: Mammalian orthoreovirus 3
UniProt:
  • Canonical: A0A0B5CSU4 (Residues: 1-1267; Coverage: 100%)
Gene name: L1
Sequence domains: Reovirus RNA-dependent RNA polymerase lambda 3
C2H2-type domain-containing protein Chains: D, E, F, G, H, I, J, K, L, M
Molecule details ›
Chains: D, E, F, G, H, I, J, K, L, M
Length: 1095 amino acids
Theoretical weight: 122.84 KDa
Source organism: Mammalian orthoreovirus 3
UniProt:
  • Canonical: F1ARN3 (Residues: 181-1275; Coverage: 86%)
Sequence domains: Inner capsid protein lambda-1/VP3
C2H2-type domain-containing protein Chains: e, g, i, k, m
Molecule details ›
Chains: e, g, i, k, m
Length: 180 amino acids
Theoretical weight: 19.11 KDa
Source organism: Mammalian orthoreovirus 3
UniProt:
  • Canonical: F1ARN3 (Residues: 1-180; Coverage: 14%)
transcript RNA Chain: C
Molecule details ›
Chain: C
Length: 4 nucleotides
Theoretical weight: 1.26 KDa
RNA (60-MER) Chains: N, O, P, Q, R, S, T, U
Molecule details ›
Chains: N, O, P, Q, R, S, T, U
Length: 60 nucleotides
Theoretical weight: 19.02 KDa

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-31183

Quick links

Citations

13 citation in other articles

Visualizing molecular interactions that determine assembly of a bullet-shaped vesicular stomatitis virus particle.
Jenni et al. (2022)
12 more

1 mention without citation

The μ2 and λ1 Proteins of Mammalian Reovirus Modulate Early Events Leading to Induction of the Interferon Signaling Network.
Després et al. (2022)