› 7elm

Electron Microscopy
2.88Å resolution

Structure of Csy-AcrIF24

Released:
DOI: 10.2210/pdb7elm/pdb
PDB entry 7elm coloured by chain, front view.
PDB entry 7elm coloured by chain, side view.
PDB entry 7elm coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Insights into the inhibition of type I-F CRISPR-Cas system by a multifunctional anti-CRISPR protein AcrIF24.
Yang L, Zhang L, Yin P, Ding H, Xiao Y, Zeng J, Wang W, Zhou H, Wang Q, Zhang Y, Chen Z, Yang M, Feng Y
Nat Commun 13 1931 (2022)
PMID: 35411005
Related structures: EMD-31185

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
PDBe Complex ID:
PDB-CPX-104785 (preferred)
Entry contents:
5 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (6 distinct):
Type I-F CRISPR-associated protein Csy1 Chains: A, K
Molecule details ›
Chains: A, K
Length: 434 amino acids
Theoretical weight: 49.31 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A3A8DDU9 (Residues: 1-434; Coverage: 100%)
Gene name: ALP65_00954
Sequence domains: CRISPR-associated protein (Cas_Csy1)
CRISPR-associated protein Csy2 Chains: B, L
Molecule details ›
Chains: B, L
Length: 327 amino acids
Theoretical weight: 36.24 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02MM0 (Residues: 1-327; Coverage: 100%)
Gene names: PA14_33320, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
CRISPR-associated protein Csy3 Chains: C, D, E, F, G, H, M, N, O, P, Q, R
Molecule details ›
Chains: C, D, E, F, G, H, M, N, O, P, Q, R
Length: 342 amino acids
Theoretical weight: 37.62 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02MM1 (Residues: 1-342; Coverage: 100%)
Gene names: PA14_33310, csy1-3, csy3
Sequence domains: CRISPR-associated protein (Cas_Csy3)
CRISPR-associated endonuclease Cas6/Csy4 Chains: I, S
Molecule details ›
Chains: I, S
Length: 187 amino acids
Theoretical weight: 21.43 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02MM2 (Residues: 1-187; Coverage: 100%)
Gene names: PA14_33300, cas6f, csy4
Sequence domains: CRISPR-associated protein (Cas_Csy4)
AcrIF24 Chains: U, V
Molecule details ›
Chains: U, V
Length: 228 amino acids
Theoretical weight: 25 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
RNA (60-MER) Chains: J, T
Molecule details ›
Chains: J, T
Length: 60 nucleotides
Theoretical weight: 19.27 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.88Å
Relevant EMDB volumes: EMD-31185
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacteriophage strategies for overcoming host antiviral immunity.
Gao et al. (2023)
3 more