7elm

Electron Microscopy
2.88Å resolution

Structure of Csy-AcrIF24

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 22-mer (preferred)
PDBe Complex ID:
PDB-CPX-104785 (preferred)
Entry contents:
5 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (6 distinct):
Type I-F CRISPR-associated protein Csy1 Chains: A, K
Molecule details ›
Chains: A, K
Length: 434 amino acids
Theoretical weight: 49.31 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A3A8DDU9 (Residues: 1-434; Coverage: 100%)
Gene name: ALP65_00954
Sequence domains: CRISPR-associated protein (Cas_Csy1)
CRISPR-associated protein Csy2 Chains: B, L
Molecule details ›
Chains: B, L
Length: 327 amino acids
Theoretical weight: 36.24 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02MM0 (Residues: 1-327; Coverage: 100%)
Gene names: PA14_33320, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
CRISPR-associated protein Csy3 Chains: C, D, E, F, G, H, M, N, O, P, Q, R
CRISPR-associated endonuclease Cas6/Csy4 Chains: I, S
AcrIF24 Chains: U, V
RNA (60-MER) Chains: J, T

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.88Å
Relevant EMDB volumes: EMD-31185
Expression system: Escherichia coli