› 7emu

X-ray diffraction
1.35Å resolution

Crystal Structure of the HasAp V37G Mutant Capturing Manganese Tetraphenylporphyrin

Released:
DOI: 10.2210/pdb7emu/pdb
PDB entry 7emu coloured by chain, front view.
PDB entry 7emu coloured by chain, side view.
PDB entry 7emu coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Tetraphenylporphyrin Enters the Ring: First Example of a Complex between Highly Bulky Porphyrins and a Protein.
Shisaka Y, Sakakibara E, Suzuki K, Stanfield JK, Onoda H, Ueda G, Hatano M, Sugimoto H, Shoji O
Chembiochem 23 e202200095 (2022)
PMID: 35352458

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme acquisition protein HasAp Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 18.79 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli M15
UniProt:
  • Canonical: G3XD33 (Residues: 1-183; Coverage: 89%)
Gene names: PA3407, hasAp
Sequence domains: Heme-binding protein A (HasA)

Ligands and Environments

2 bound ligands:
Ligand J7X 1 x J7X
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 51.037Å b: 62.233Å c: 63.383Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.192
Expression system: Escherichia coli M15

Quick links

Citations

1 citations in other articles

Identification of Blood Transport Proteins to Carry Temoporfin: A Domino Approach from Virtual Screening to Synthesis and In Vitro PDT Testing.
Marconi et al. (2023)

1 mention without citation

Preparation of core-shell structured magnetic covalent organic framework nanocomposites for magnetic solid-phase extraction of bisphenols from human serum sample.
Chen et al. (2018)