› 7eow

X-ray diffraction
1.6Å resolution

High-resolution structure of vWF A1 domain in complex with caplacizumab, the first nanobody-based medicine

Released:
DOI: 10.2210/pdb7eow/pdb
Model geometry
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PDB entry 7eow coloured by chain, front view.
PDB entry 7eow coloured by chain, side view.
PDB entry 7eow coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-resolution structure of the vWF A1 domain in complex with caplacizumab, the first nanobody-based medicine for treating acquired TTP.
Lee HT, Park UB, Jeong TJ, Gu N, Lee SH, Kim Y, Heo YS
Biochem Biophys Res Commun 567 49-55 (2021)
PMID: 34144500

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209006 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
von Willebrand factor Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 26.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04275 (Residues: 1261-1468; Coverage: 8%)
Gene names: F8VWF, VWF
Sequence domains: von Willebrand factor type A domain
caplacizumab Chain: B
Molecule details ›
Chain: B
Length: 137 amino acids
Theoretical weight: 15.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P61
Unit cell:
a: 74.741Å b: 74.741Å c: 122.288Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.175 0.198
Expression system: Escherichia coli

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Citations

1 review citation

Discovery of nanobodies: a comprehensive review of their applications and potential over the past five years.
Alexander et al. (2024)
5 other articles

3 mentions without citation

Von Willebrand factor: A key glycoprotein involved in thrombo-inflammatory complications of COVID-19.
Choudhary et al. (2021)
2 more

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