› 7esz

X-ray diffraction
2.48Å resolution

Crystal structure of the complex formed by Wolbachia cytoplasmic incompatibility factors CinA and CinB with Mn2+ from wPip

Released:
DOI: 10.2210/pdb7esz/pdb
PDB entry 7esz coloured by chain, front view.
PDB entry 7esz coloured by chain, side view.
PDB entry 7esz coloured by chain, top view.
Source organism: Wolbachia endosymbiont of Culex quinquefasciatus Pel
Primary publication:
Structural and mechanistic insights into the complexes formed by Wolbachia cytoplasmic incompatibility factors.
Xiao Y, Chen H, Wang H, Zhang M, Chen X, Berk JM, Zhang L, Wei Y, Li W, Cui W, Wang F, Wang Q, Cui C, Li T, Chen C, Ye S, Zhang L, Ji X, Huang J, Wang W, Wang Z, Hochstrasser M, Yang H
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34620712

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241542 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
BACTERIA FACTOR B Chains: A, C
Molecule details ›
Chains: A, C
Length: 740 amino acids
Theoretical weight: 84.33 KDa
Source organism: Wolbachia endosymbiont of Culex quinquefasciatus Pel
Expression system: Escherichia coli
BACTERIA FACTOR A Chains: B, D
Molecule details ›
Chains: B, D
Length: 453 amino acids
Theoretical weight: 52.6 KDa
Source organism: Wolbachia endosymbiont of Culex quinquefasciatus Pel
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P1
Unit cell:
a: 85.466Å b: 86.38Å c: 93.018Å
α: 110.06° β: 91.86° γ: 115.38°
R-values:
R R work R free
0.201 0.198 0.25
Expression system: Escherichia coli

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Citations

2 review citations

Management of the poultry red mite Dermanyssus gallinae with physical control methods by inorganic material and future perspectives.
Hwang ET. (2023)
1 more