› 7ex4

X-ray diffraction
2Å resolution

Crystal structure of Ebinur Lake virus cap snatching endonuclease in complex with inhibitor 5

Released:
DOI: 10.2210/pdb7ex4/pdb
PDB entry 7ex4 coloured by chain, front view.
PDB entry 7ex4 coloured by chain, side view.
PDB entry 7ex4 coloured by chain, top view.
Source organism: Ebinur lake virus
Primary publication:
Structural and Biochemical Basis for Development of Diketo Acid Inhibitors Targeting the Cap-Snatching Endonuclease of the Ebinur Lake Virus (Order: Bunyavirales).
Kuang W, Zhang H, Cai Y, Zhang G, Deng F, Li H, Zhou Y, Wang M, Gong P, Guo Y, Hu Z
J Virol 96 e0217321 (2022)
PMID: 35266805

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-492343 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase L Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 25.18 KDa
Source organism: Ebinur lake virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A059WLS9 (Residues: 1-183; Coverage: 8%)
Gene name: RdRp
Sequence domains: L protein N-terminus

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand IA5 1 x IA5
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 40.933Å b: 70.566Å c: 70.882Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Antiviral Activity of Acetylsalicylic Acid against Bunyamwera Virus in Cell Culture.
Fernández-Sánchez et al. (2023)

1 mention without citation

Structural and Biochemical Basis for Development of Diketo Acid Inhibitors Targeting the Cap-Snatching Endonuclease of the Ebinur Lake Virus (Order: Bunyavirales).
Kuang et al. (2022)