› 7exk

X-ray diffraction
2.14Å resolution

Function and Biology Details

Reaction catalysed: 
((1->4)-beta-D-glucosyl)(n+m) + reduced acceptor + O(2) = 4-dehydro-beta-D-glucosyl-((1->4)-beta-D-glucosyl)(n-1) + ((1->4)-beta-D-glucosyl)(m) + acceptor + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125975 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
lytic cellulose monooxygenase (C4-dehydrogenating) Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 217 amino acids
Theoretical weight: 22.94 KDa
Source organism: Gelatoporia subvermispora B
Expression system: Komagataella pastoris
UniProt:
  • Canonical: M2RAI8 (Residues: 19-235; Coverage: 100%)
Gene name: CERSUDRAFT_85414
Sequence domains: Auxiliary Activity family 9 (formerly GH61)

Ligands and Environments

6 bound ligands:
Ligand NAG 6 x NAG
Ligand CU 6 x CU
Ligand TRS 6 x TRS
Ligand EDO 66 x EDO
Ligand XYS 1 x XYS
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 84.063Å b: 52.428Å c: 134.625Å
α: 90° β: 90.553° γ: 90°
R-values:
R R work R free
0.175 0.172 0.23
Expression system: Komagataella pastoris

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