› 7exs

X-ray diffraction
1.42Å resolution

Thermomicrobium roseum sarcosine oxidase mutant - S320R

Released:
DOI: 10.2210/pdb7exs/pdb
PDB entry 7exs coloured by chain, front view.
PDB entry 7exs coloured by chain, side view.
PDB entry 7exs coloured by chain, top view.
Source organism: Thermomicrobium roseum DSM 5159
Primary publication:
Recreating the natural evolutionary trend in key microdomains provides an effective strategy for engineering of a thermomicrobial N-demethylase.
Xin Y, Shen C, Tang M, Guo Z, Shi Y, Gu Z, Shao J, Zhang L
J Biol Chem 298 101656 (2022)
PMID: 35124004

Function and Biology Details

Reaction catalysed: 
Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD dependent oxidoreductase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 372 amino acids
Theoretical weight: 41.38 KDa
Source organism: Thermomicrobium roseum DSM 5159
Expression system: Bacillus subtilis
UniProt:
  • Canonical: B9L163 (Residues: 6-377; Coverage: 98%)
Gene name: trd_1773
Sequence domains: FAD dependent oxidoreductase

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
3 bound ligands:
Ligand GOL 6 x GOL
Ligand CL 7 x CL
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 130.362Å b: 47.141Å c: 64.763Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.217
Expression system: Bacillus subtilis

Quick links

Citations

1 mention without citation

Recreating the natural evolutionary trend in key microdomains provides an effective strategy for engineering of a thermomicrobial N-demethylase.
Xin et al. (2022)