› 7f07

X-ray diffraction
2.25Å resolution

Autonomous VH domain that interacts with eIF4E at the Capped mRNA Binding site.

Released:
DOI: 10.2210/pdb7f07/pdb
PDB entry 7f07 coloured by chain, front view.
PDB entry 7f07 coloured by chain, side view.
PDB entry 7f07 coloured by chain, top view.
Source organisms:
Primary publication:
Development of a novel peptide aptamer that interacts with the eIF4E capped-mRNA binding site using peptide epitope linker evolution (PELE).
Frosi Y, Ng S, Lin YC, Jiang S, Ramlan SR, Lama D, Verma CS, Asial I, Brown CJ
OpenAccess logo RSC Chem Biol 3 916-930 (2022)
PMID: 35866173

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225082 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 25.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06730 (Residues: 1-217; Coverage: 100%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
VH domain (VH-DiFCAP-01) Chain: C
Molecule details ›
Chain: C
Length: 179 amino acids
Theoretical weight: 19.11 KDa
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 38.522Å b: 81.819Å c: 122.987Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.266
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Methanogen diversity evidenced by molecular characterization of methyl coenzyme M reductase A (mcrA) genes in hydrothermal sediments of the Guaymas Basin.
Dhillon et al. (2005)