› 7f0b

X-ray diffraction
2.14Å resolution

Crystal structure of capreomycin phosphotransferase in complex with ATP

Released:
DOI: 10.2210/pdb7f0b/pdb
PDB entry 7f0b coloured by chain, front view.
PDB entry 7f0b coloured by chain, side view.
PDB entry 7f0b coloured by chain, top view.
Source organism: Saccharothrix mutabilis subsp. capreolus
Primary publication:
Dual-Mechanism Confers Self-Resistance to the Antituberculosis Antibiotic Capreomycin.
Pan YC, Wang YL, Toh SI, Hsu NS, Lin KH, Xu Z, Huang SC, Wu TK, Li TL, Chang CY
ACS Chem Biol 17 138-146 (2022)
PMID: 34994196

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176080 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 31.44 KDa
Source organism: Saccharothrix mutabilis subsp. capreolus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53826 (Residues: 1-281; Coverage: 100%)
Gene name: cph
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

1 bound ligand:
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P4222
Unit cell:
a: 91.833Å b: 91.833Å c: 120.619Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.251
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Newly Discovered Mechanisms of Antibiotic Self-Resistance with Multiple Enzymes Acting at Different Locations and Stages.
Chen et al. (2022)
1 other article

1 mention without citation

Discovery and characterization of genes conferring natural resistance to the antituberculosis antibiotic capreomycin.
Toh et al. (2023)