› 7f0f

X-ray diffraction
2.1Å resolution

Crystal structure of capreomycin phosphotransferase in complex with CMN IIB

Released:
DOI: 10.2210/pdb7f0f/pdb
Model geometry
Fit model/data
PDB entry 7f0f coloured by chain, front view.
PDB entry 7f0f coloured by chain, side view.
PDB entry 7f0f coloured by chain, top view.
Source organism: Saccharothrix mutabilis subsp. capreolus
Primary publication:
Dual-Mechanism Confers Self-Resistance to the Antituberculosis Antibiotic Capreomycin.
Pan YC, Wang YL, Toh SI, Hsu NS, Lin KH, Xu Z, Huang SC, Wu TK, Li TL, Chang CY
ACS Chem Biol 17 138-146 (2022)
PMID: 34994196

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168262 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aminoglycoside phosphotransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 31.44 KDa
Source organism: Saccharothrix mutabilis subsp. capreolus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53826 (Residues: 1-281; Coverage: 100%)
Gene name: cph
Sequence domains: Phosphotransferase enzyme family
DPP-ALA-DPP-UAL-MYN-KBE Chain: C
Molecule details ›
Chain: C
Length: 6 amino acids
Theoretical weight: 671 Da
Source organism: Saccharothrix mutabilis subsp. capreolus
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
3 modified residues:
Ligand KBE 1 x KBE
Ligand DPP 2 x DPP
Ligand MYN 1 x MYN

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P4222
Unit cell:
a: 92.101Å b: 92.101Å c: 119.006Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.223
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Newly Discovered Mechanisms of Antibiotic Self-Resistance with Multiple Enzymes Acting at Different Locations and Stages.
Chen et al. (2022)
2 other articles

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO