Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- RNA polymerase sigma-70
- DNA-directed RNA polymerase, insert domain superfamily
- DNA-directed RNA polymerase, RpoA/D/Rpb3-type
- RNA polymerase, RBP11-like subunit
- DNA-directed RNA polymerase, alpha subunit
- DNA-directed RNA polymerase, omega subunit
- Winged helix-like DNA-binding domain superfamily
- RNA polymerase sigma factor, region 3/4-like
36 more domains
Structure analysis Details
Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-121273 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 38.26 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 345 amino acids
Theoretical weight: 38.26 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
O52760 (Residues: 1-333; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit beta
Molecule details ›
Chain: C
Length: 1359 amino acids
Theoretical weight: 151.23 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 1359 amino acids
Theoretical weight: 151.23 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q51561 (Residues: 1-1357; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit beta'
Molecule details ›
Chain: D
Length: 1412 amino acids
Theoretical weight: 156.04 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 1412 amino acids
Theoretical weight: 156.04 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q9HWC9 (Residues: 2-1399; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit omega
Molecule details ›
Chain: E
Length: 88 amino acids
Theoretical weight: 9.78 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: RNA polymerase Rpb6
Length: 88 amino acids
Theoretical weight: 9.78 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q9HTM1 (Residues: 1-88; Coverage: 100%)
Sequence domains: RNA polymerase Rpb6
Transcriptional regulator SutA RNAP-binding domain-containing protein
Molecule details ›
Chain: G
Length: 109 amino acids
Theoretical weight: 11.5 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: SutA RNAP-binding domain
Length: 109 amino acids
Theoretical weight: 11.5 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q9HTR9 (Residues: 1-105; Coverage: 100%)
Sequence domains: SutA RNAP-binding domain
RNA polymerase sigma factor RpoS
Molecule details ›
Chain: F
Length: 342 amino acids
Theoretical weight: 39.36 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 342 amino acids
Theoretical weight: 39.36 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P45684 (Residues: 1-334; Coverage: 100%)
Sequence domains:
