› 7f2f

X-ray diffraction
2.55Å resolution

The complex of DNA with the C-terminal domain of TYE7 from Saccharomyces cerevisiae.

Released:
DOI: 10.2210/pdb7f2f/pdb
PDB entry 7f2f coloured by chain, front view.
PDB entry 7f2f coloured by chain, side view.
PDB entry 7f2f coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the complex of DNA with the C-terminal domain of TYE7 from Saccharomyces cerevisiae.
Gui W, Xue L, Yue J, Kuang Z, Jin Y, Niu L
Acta Crystallogr F Struct Biol Commun 77 341-347 (2021)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121274 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor TYE7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 136 amino acids
Theoretical weight: 15.86 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P33122 (Residues: 165-291; Coverage: 44%)
Gene names: O6233, SGC1, TYE7, YOR344C
Sequence domains: Helix-loop-helix DNA-binding domain
DNA (5'-D(*CP*AP*GP*AP*TP*CP*AP*TP*GP*TP*GP*TP*GP*CP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*GP*GP*GP*CP*AP*CP*AP*CP*AP*TP*GP*AP*TP*CP*T)-3') Chain: H
Molecule details ›
Chain: H
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P41212
Unit cell:
a: 73.025Å b: 73.025Å c: 181.782Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.246 0.284
Expression systems:
  • Escherichia coli
  • Not provided

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