› 7f3e

Electron Microscopy
3.62Å resolution

Cryo-EM structure of the minimal protein-only RNase P from Aquifex aeolicus

Released:
DOI: 10.2210/pdb7f3e/pdb
PDB entry 7f3e coloured by chain, front view.
PDB entry 7f3e coloured by chain, side view.
PDB entry 7f3e coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Minimal protein-only RNase P structure reveals insights into tRNA precursor recognition and catalysis.
Teramoto T, Koyasu T, Adachi N, Kawasaki M, Moriya T, Numata T, Senda T, Kakuta Y
OpenAccess logo J Biol Chem 297 101028 (2021)
PMID: 34339732
Related structures: EMD-31432

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130414 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-free ribonuclease P Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 195 amino acids
Theoretical weight: 22.82 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67035 (Residues: 1-192; Coverage: 100%)
Gene name: aq_880
Sequence domains: PINc domain ribonuclease

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.62Å
Relevant EMDB volumes: EMD-31432
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The Dynamic Network of RNP RNase P Subunits.
Shaukat et al. (2021)
2 more

2 mentions without citation

Minimal protein-only RNase P structure reveals insights into tRNA precursor recognition and catalysis.
Teramoto et al. (2021)
1 more