› 7f3q

Electron Microscopy
3.3Å resolution

SARS-CoV-2 RBD in complex with A5-10 Fab and A34-2 Fab

Released:
DOI: 10.2210/pdb7f3q/pdb
PDB entry 7f3q coloured by chain, front view.
PDB entry 7f3q coloured by chain, side view.
PDB entry 7f3q coloured by chain, top view.
Source organisms:
Primary publication:
Development of neutralizing antibodies against SARS-CoV-2, using a high-throughput single-B-cell cloning method.
Dou Y, Xu K, Deng YQ, Jia Z, Lan J, Xu X, Zhang G, Cao T, Liu P, Wang X, Wang X, Xu L, Du P, Qin CF, Liu H, Li Y, Wu G, Wang K, Lu B
OpenAccess logo Antib Ther 6 76-86 (2023)
PMID: 37077472
Related structures: EMD-31434

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209762 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Spike glycoprotein Chain: A
Molecule details ›
Chain: A
Length: 1273 amino acids
Theoretical weight: 141.3 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 1-1273; Coverage: 100%)
Gene names: 2, S
Sequence domains:
Heavy chain of A34-2 Fab Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.33 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Light chain of A34-2 Fab Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 22.77 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Heavy chain of A5-10 Fab Chain: C
Molecule details ›
Chain: C
Length: 227 amino acids
Theoretical weight: 24.01 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Light chain of A5-10 Fab Chain: B
Molecule details ›
Chain: B
Length: 205 amino acids
Theoretical weight: 22.18 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

2 bound ligands:
Ligand NAG 1 x NAG
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-31434
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein.
Li et al. (2024)