› 7f51

X-ray diffraction
1.98Å resolution

Crystal structure of Hst2 in complex with 2'-O-Benzoyl ADP Ribose

Released:
DOI: 10.2210/pdb7f51/pdb
PDB entry 7f51 coloured by chain, front view.
PDB entry 7f51 coloured by chain, side view.
PDB entry 7f51 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Global profiling of regulatory elements in the histone benzoylation pathway.
Wang D, Yan F, Wu P, Ge K, Li M, Li T, Gao Y, Peng C, Chen Y
OpenAccess logo Nat Commun 13 1369 (2022)
PMID: 35296687

Function and Biology Details

Reaction catalysed: 
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156852 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent protein deacetylase HST2 Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 32.52 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P53686 (Residues: 8-294; Coverage: 80%)
Gene names: HST2, LPA2C, YPL015C
Sequence domains: Sir2 family

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand BA7 1 x BA7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 42.166Å b: 67.363Å c: 92.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.22
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Protein posttranslational modifications in health and diseases: Functions, regulatory mechanisms, and therapeutic implications.
Zhong et al. (2023)
2 more