› 7f58

Electron Microscopy
3.1Å resolution

Cryo-EM structure of THIQ-MC4R-Gs_Nb35 complex

Released:
DOI: 10.2210/pdb7f58/pdb
PDB entry 7f58 coloured by chain, front view.
PDB entry 7f58 coloured by chain, side view.
PDB entry 7f58 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into ligand recognition and activation of the melanocortin-4 receptor.
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res 31 1163-1175 (2021)
PMID: 34433901
Related structures: EMD-31461

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-211061 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Melanocortin receptor 4 Chain: R
Molecule details ›
Chain: R
Length: 507 amino acids
Theoretical weight: 56.05 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P32245 (Residues: 1-332; Coverage: 100%)
Gene name: MC4R
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 96%)
  • Best match: P63092-2 (Residues: 1-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 384 amino acids
Theoretical weight: 41.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody35 Chain: N
Molecule details ›
Chain: N
Length: 126 amino acids
Theoretical weight: 13.71 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand 1I8 1 x 1I8
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-31461
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 review citation

Lipocalin 2 receptors: facts, fictions, and myths.
Schröder et al. (2023)
14 other articles

3 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
2 more