› 7f66

Electron Microscopy
2.76Å resolution

eIF2B-SFSV NSs-1-eIF2

Released:
DOI: 10.2210/pdb7f66/pdb
PDB entry 7f66 coloured by chain, front view.
PDB entry 7f66 coloured by chain, side view.
PDB entry 7f66 coloured by chain, top view.
Source organisms:
Primary publication:
eIF2B-capturing viral protein NSs suppresses the integrated stress response.
Kashiwagi K, Shichino Y, Osaki T, Sakamoto A, Nishimoto M, Takahashi M, Mito M, Weber F, Ikeuchi Y, Iwasaki S, Ito T
Nat Commun 12 7102 (2021)
PMID: 34876589
Related structures: EMD-31474

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138584 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Translation initiation factor eIF2B subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 33.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14232 (Residues: 1-305; Coverage: 100%)
Gene names: EIF2B1, EIF2BA
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 351 amino acids
Theoretical weight: 39.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49770 (Residues: 1-351; Coverage: 100%)
Gene names: EIF2B2, EIF2BB
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit gamma Chains: E, F
Molecule details ›
Chains: E, F
Length: 452 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR50 (Residues: 1-452; Coverage: 100%)
Gene name: EIF2B3
Sequence domains: Nucleotidyl transferase
Translation initiation factor eIF2B subunit delta Chains: G, H
Molecule details ›
Chains: G, H
Length: 523 amino acids
Theoretical weight: 57.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI10 (Residues: 1-523; Coverage: 100%)
Gene names: EIF2B4, EIF2BD
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit epsilon Chains: I, J
Molecule details ›
Chains: I, J
Length: 721 amino acids
Theoretical weight: 80.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13144 (Residues: 1-721; Coverage: 100%)
Gene names: EIF2B5, EIF2BE
Sequence domains: eIF4-gamma/eIF5/eIF2-epsilon
Non-structural protein NS-S Chains: K, L
Molecule details ›
Chains: K, L
Length: 261 amino acids
Theoretical weight: 29.7 KDa
Source organism: sandfly fever Sicilian virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P12792 (Residues: 7-267; Coverage: 98%)
Gene name: NSS
Sequence domains: Rift valley fever virus non structural protein (NSs) like
Eukaryotic translation initiation factor 2 subunit 1 Chain: N
Molecule details ›
Chain: N
Length: 315 amino acids
Theoretical weight: 36.16 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P05198 (Residues: 1-315; Coverage: 100%)
Gene names: EIF2A, EIF2S1
Sequence domains:
eIF2beta Chain: O
Molecule details ›
Chain: O
Length: 14 amino acids
Theoretical weight: 1.21 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Eukaryotic translation initiation factor 2 subunit 3 Chain: S
Molecule details ›
Chain: S
Length: 472 amino acids
Theoretical weight: 51.18 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P41091 (Residues: 1-472; Coverage: 100%)
Gene names: EIF2G, EIF2S3
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.76Å
Relevant EMDB volumes: EMD-31474
Expression systems:
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

3 review citations

Regulation and function of elF2B in neurological and metabolic disorders.
Hanson et al. (2022)
2 more

1 mention without citation

Protection of eIF2B from inhibitory phosphorylated eIF2: A viral strategy to maintain mRNA translation during the PKR-triggered integrated stress response.
Ito et al. (2023)