› 7f6h

Electron Microscopy
2.9Å resolution

Cryo-EM structure of human bradykinin receptor BK2R in complex Gq proteins and bradykinin

Released:
DOI: 10.2210/pdb7f6h/pdb
PDB entry 7f6h coloured by chain, front view.
PDB entry 7f6h coloured by chain, side view.
PDB entry 7f6h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures of human bradykinin receptor-Gq proteins complexes.
Shen J, Zhang D, Fu Y, Chen A, Yang X, Zhang H
OpenAccess logo Nat Commun 13 714 (2022)
PMID: 35132089
Related structures: EMD-31480

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-133902 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
B2 bradykinin receptor Chain: A
Molecule details ›
Chain: A
Length: 770 amino acids
Theoretical weight: 86.46 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P30411 (Residues: 25-391; Coverage: 94%)
  • Best match: P30411-2 (Residues: 2-364)
Gene names: BDKRB2, BKR2
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Bradykinin Chain: L
Molecule details ›
Chain: L
Length: 9 amino acids
Theoretical weight: 1.06 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01042 (Residues: 381-389; Coverage: 1%)
Gene names: BDK, KNG, KNG1
Guanine nucleotide-binding protein G(q) subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 369 amino acids
Theoretical weight: 43.53 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P50148 (Residues: 2-359; Coverage: 100%)
Gene names: GAQ, GNAQ
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: C
Molecule details ›
Chain: C
Length: 354 amino acids
Theoretical weight: 39.09 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: D
Molecule details ›
Chain: D
Length: 81 amino acids
Theoretical weight: 9.24 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 2-71; Coverage: 99%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

1 bound ligand:
Ligand CLR 2 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-31480
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

3 review citations

Gq Signaling in Autophagy Control: Between Chemical and Mechanical Cues.
Navarro-Lérida et al. (2022)
2 more

3 mentions without citation

Function and structure of bradykinin receptor 2 for drug discovery.
Shen et al. (2023)
2 more