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Cryo-EM structure of the human TACAN in a closed state.

Chen X, Wang Y, Li Y, Lu X, Chen J, Li M, Wen T, Liu N, Chang S, Zhang X, Yang X, Shen Y
Cell Rep 38 110445 (2022)
Cited: 10 times
EuropePMC logo PMID: 35235791

Abstract

TACAN is an ion channel-like protein that may be involved in sensing mechanical pain. Here, we present the cryo-electron microscopic structure of human TACAN (hTACAN). hTACAN forms a dimer in which each protomer consists of a transmembrane globular domain (TMD) containing six helices and an intracellular domain (ICD) containing two helices. Molecular dynamic simulations suggest that each protomer contains a putative ion conduction pore. A single-point mutation of the key residue Met207 greatly increases membrane pressure-activated currents. In addition, each hTACAN subunit binds one cholesterol molecule. Our data show the molecular assembly of hTACAN and suggest that wild-type hTACAN is in a closed state.

Reviews - 7f6v mentioned but not cited (1)

  1. Transmembrane proteins with unknown function (TMEMs) as ion channels: electrophysiological properties, structure, and pathophysiological roles. Kang H, Lee CJ. Exp Mol Med 56 850-860 (2024)


Reviews citing this publication (3)

  1. Force From Filaments: The Role of the Cytoskeleton and Extracellular Matrix in the Gating of Mechanosensitive Channels. Chuang YC, Chen CC. Front Cell Dev Biol 10 886048 (2022)
  2. TMEM120A/TACAN: A putative regulator of ion channels, mechanosensation, and lipid metabolism. Gabrielle M, Rohacs T. Channels (Austin) 17 2237306 (2023)
  3. To Be or Not to Be an Ion Channel: Cryo-EM Structures Have a Say. Chen GL, Li J, Zhang J, Zeng B. Cells 12 1870 (2023)

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