› 7f8r

X-ray diffraction
2.51Å resolution

Crystal structure of human soluble CLIC1 with catalytic cysteine (Cys24) in sulphonic acid form.

Released:
DOI: 10.2210/pdb7f8r/pdb
PDB entry 7f8r coloured by chain, front view.
PDB entry 7f8r coloured by chain, side view.
PDB entry 7f8r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Comparative kinetic analysis of ascorbate (Vitamin-C) recycling dehydroascorbate reductases from plants and humans.
Das BK, Kumar A, Sreekumar SN, Ponraj K, Gadave K, Kumar S, Murali Achary VM, Ray P, Reddy MK, Arockiasamy A
Biochem Biophys Res Commun 591 110-117 (2022)
PMID: 35007834

Function and Biology Details

Reaction catalysed: 
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-517204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloride intracellular channel protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 241 amino acids
Theoretical weight: 27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00299 (Residues: 1-241; Coverage: 100%)
Gene names: CLIC1, G6, NCC27
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand OCS 2 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 42.02Å b: 70.655Å c: 82.412Å
α: 90° β: 90.13° γ: 90°
R-values:
R R work R free
0.178 0.175 0.236
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

Prediction of Apple Slices Drying Kinetic during Infrared-Assisted-Hot Air Drying by Deep Neural Networks.
Huang et al. (2022)