› 7f90

X-ray diffraction
2.39Å resolution

Crystal structure of SARS auxiliary protein in complex with human nuclear protein

Released:
DOI: 10.2210/pdb7f90/pdb
PDB entry 7f90 coloured by chain, front view.
PDB entry 7f90 coloured by chain, side view.
PDB entry 7f90 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for Sarbecovirus ORF6 mediated blockage of nucleocytoplasmic transport
Gao X, Tian H, Zhu K, Li Q, Hao W, Wang L, Qin B, Deng H, Cui S
Nat Commun 13 4782- (2022)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-156677 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ORF6 protein Chains: E, F
Molecule details ›
Chains: E, F
Length: 63 amino acids
Theoretical weight: 7.53 KDa
Source organism: Severe acute respiratory syndrome-related coronavirus
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P59634 (Residues: 1-63; Coverage: 100%)
Gene name: 6
Sequence domains: Betacoronavirus NS6 protein
mRNA export factor RAE1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 368 amino acids
Theoretical weight: 41.02 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P78406 (Residues: 1-368; Coverage: 100%)
Gene names: MRNP41, RAE1
Sequence domains: WD domain, G-beta repeat
Nuclear pore complex protein Nup98-Nup96 Chains: B, D
Molecule details ›
Chains: B, D
Length: 1817 amino acids
Theoretical weight: 197.78 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P52948 (Residues: 1-1817; Coverage: 100%)
Gene names: ADAR2, NUP98
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 189.332Å b: 86.794Å c: 47.905Å
α: 90° β: 92.09° γ: 90°
R-values:
R R work R free
0.222 0.22 0.258
Expression system: Insect cell expression vector pTIE1

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