› 7f97

X-ray diffraction
2.39Å resolution

Plasmodium falciparum Prolyl-tRNA Synthetase (PfPRS) in Complex with L-proline and compound L97

Released:
DOI: 10.2210/pdb7f97/pdb
PDB entry 7f97 coloured by chain, front view.
PDB entry 7f97 coloured by chain, side view.
PDB entry 7f97 coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
ATP mimetics targeting prolyl-tRNA synthetases as a new avenue for antimalarial drug development
Mishra S, Malhotra N, Laleu B, Chakraborti S, Yogavel M, Sharma A
Iscience 27 (2024)

Function and Biology Details

Reaction catalysed: 
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proline--tRNA ligase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 497 amino acids
Theoretical weight: 57.8 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8I5R7 (Residues: 254-746; Coverage: 66%)
Gene names: PF3D7_1213800, PFL0670c, PRS, proRS
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand PRO 4 x PRO
Ligand 1XK 4 x 1XK
Ligand BU1 1 x BU1
Ligand CL 10 x CL
Ligand HEZ 1 x HEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 82.02Å b: 140.6Å c: 187.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.232
Expression system: Escherichia coli

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