› 7fe0

X-ray diffraction
2.2Å resolution

AvmM Catalyzes Macrocyclization in Alchivemycin A Biosynthesis

Released:
DOI: 10.2210/pdb7fe0/pdb
PDB entry 7fe0 coloured by chain, front view.
PDB entry 7fe0 coloured by chain, side view.
PDB entry 7fe0 coloured by chain, top view.
Source organism: Streptomyces sp. NBRC 109436
Primary publication:
AvmM catalyses macrocyclization through dehydration/Michael-type addition in alchivemycin A biosynthesis.
Zhu HJ, Zhang B, Wei W, Liu SH, Xiang L, Zhu J, Jiao RH, Igarashi Y, Bashiri G, Liang Y, Tan RX, Ge HM
OpenAccess logo Nat Commun 13 4499 (2022)
PMID: 35922406

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-498842 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AvmM Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 217 amino acids
Theoretical weight: 24.14 KDa
Source organism: Streptomyces sp. NBRC 109436
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A8F7LEJ5 (Residues: 1-197; Coverage: 100%)
Gene name: avmM

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand CAC 1 x CAC
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P41212
Unit cell:
a: 73.87Å b: 73.87Å c: 243.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.22
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

Development of a drug discovery approach from microbes with a special focus on isolation sources and taxonomy.
Igarashi Y. (2023)
3 other articles

2 mentions without citation

A DICOM dataset for evaluation of medical image de-identification.
Rutherford et al. (2021)
1 more