› 7fgm

X-ray diffraction
2.2Å resolution

The complex crystals structure of the FAF1 UBL1_L-Hsp70 NBD with ADP and phosphate

Released:
DOI: 10.2210/pdb7fgm/pdb
PDB entry 7fgm coloured by chain, front view.
PDB entry 7fgm coloured by chain, side view.
PDB entry 7fgm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The complex of Fas-associated factor 1 with Hsp70 stabilizes the adherens junction integrity by suppressing RhoA activation
Song S, Park JK, Shin SC, Lee JJ, Hong SK, Song IK, Kim B, Song EJ, Lee KJ, Kim EE
J Mol Cell Biol 14 (2022)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143653 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heat shock 70 kDa protein 1A Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 42.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DMV8 (Residues: 1-382; Coverage: 60%)
Gene names: HSP72, HSPA1, HSPA1A, HSX70
Sequence domains: Hsp70 protein
FAS-associated factor 1 Chain: B
Molecule details ›
Chain: B
Length: 81 amino acids
Theoretical weight: 9.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UNN5 (Residues: 91-171; Coverage: 13%)
Gene names: CGI-03, FAF1, UBXD12, UBXN3A

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P3121
Unit cell:
a: 103.335Å b: 103.335Å c: 127.093Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.206
Expression system: Escherichia coli

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