› 7fh2

X-ray diffraction
2.49Å resolution

Crystal structure of the first bromodomain of BRD4 in complex with 16D10

Released:
DOI: 10.2210/pdb7fh2/pdb
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PDB entry 7fh2 coloured by chain, front view.
PDB entry 7fh2 coloured by chain, side view.
PDB entry 7fh2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A chalcone derivative suppresses TSLP induction in mice and human keratinocytes through binding to BET family proteins.
Segawa R, Takeda H, Yokoyama T, Ishida M, Miyata C, Saito T, Ishihara R, Nakagita T, Sasano Y, Kanoh N, Iwabuchi Y, Mizuguchi M, Hiratsuka M, Hirasawa N
Biochem Pharmacol 194 114819 (2021)
PMID: 34757034

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bromodomain-containing protein 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 135 amino acids
Theoretical weight: 16.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60885 (Residues: 42-168; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain

Ligands and Environments

2 bound ligands:
Ligand 4JI 4 x 4JI
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 73.01Å b: 75.876Å c: 110.803Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.253 0.292
Expression system: Escherichia coli

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Citations

1 citations in other articles

Evaluation of Cell-Free Synthesized Human Channel Proteins for In Vitro Channel Research.
Nishiguchi et al. (2022)

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