› 7fho

Electron Microscopy
2.8Å resolution

Structure of AtTPC1 D240A/D454A/E528A mutant with 50 mM Ca2+

Released:
DOI: 10.2210/pdb7fho/pdb
PDB entry 7fho coloured by chain, front view.
PDB entry 7fho coloured by chain, side view.
PDB entry 7fho coloured by chain, top view.
Source organisms:
Primary publication:
Voltage-gating and cytosolic Ca2+ activation mechanisms of Arabidopsis two-pore channel AtTPC1.
Ye F, Xu L, Li X, Zeng W, Gan N, Zhao C, Yang W, Jiang Y, Guo J
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34845029
Related structures: EMD-31588

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154525 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; Two pore calcium channel protein 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 998 amino acids
Theoretical weight: 114.5 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: Q94KI8 (Residues: 1-733; Coverage: 100%)
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene names: At4g03560, CCH1, F9H3.19, FOU2, GFP, T5L23.5, TPC1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-31588
Expression system: Homo sapiens

Quick links

Citations

4 review citations

Calcium channels and transporters: Roles in response to biotic and abiotic stresses.
Park et al. (2022)
3 more

1 mention without citation

Structural biology of cation channels important for lysosomal calcium release.
Gan et al. (2022)