Function and Biology Details
Reactions catalysed:
Cleaves hyaluronate chains at a beta-D-GlcNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
Eliminative cleavage of (1->4)-beta-D-glucuronans to give oligosaccharides with 4-deoxy-beta-D-gluc-4-enuronosyl groups at their non-reducing ends. Complete degradation of glucuronans results in the formation of tetrasaccharides.
Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.
Biochemical function:
Biological process:
- not assigned
Cellular component:
Sequence domains:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
Polysaccharide lyase (preferred)
PDBe Complex ID:
PDB-CPX-109508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Polysaccharide lyase
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 35.43 KDa
Source organism: Stenotrophomonas maltophilia K279a
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: Alginate lyase
Length: 316 amino acids
Theoretical weight: 35.43 KDa
Source organism: Stenotrophomonas maltophilia K279a
Expression system: Escherichia coli BL21
UniProt:
- Canonical:
B2FHL8 (Residues: 22-331; Coverage: 100%)
Sequence domains: Alginate lyase
