› 7g86

X-ray diffraction
1.7Å resolution

ARHGEF2 PanDDA analysis group deposition -- ARHGEF2 and RhoA in complex with Z1137725943

Released:
DOI: 10.2210/pdb7g86/pdb
PDB entry 7g86 coloured by chain, front view.
PDB entry 7g86 coloured by chain, side view.
PDB entry 7g86 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Bradshaw WJ, Katis VL, Bountra C, von Delft F, Brennan PE

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-215193 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transforming protein RhoA Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 20.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61586 (Residues: 1-184; Coverage: 95%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Rho guanine nucleotide exchange factor 2 Chain: B
Molecule details ›
Chain: B
Length: 245 amino acids
Theoretical weight: 28.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92974 (Residues: 206-448; Coverage: 25%)
Gene names: ARHGEF2, KIAA0651, LFP40
Sequence domains: RhoGEF domain

Ligands and Environments

3 bound ligands:
Ligand YXB 3 x YXB
Ligand DMS 6 x DMS
Ligand FMT 10 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P43212
Unit cell:
a: 71.4Å b: 71.4Å c: 197.117Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.229
Expression system: Escherichia coli

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