› 7gnz

X-ray diffraction
1.61Å resolution

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-280226 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease 3C Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.13 KDa
Source organism: enterovirus D68
Expression system: Escherichia coli
UniProt:
  • Canonical: Q68T42 (Residues: 1548-1729; Coverage: 8%)
Sequence domains: 3C cysteine protease (picornain 3C)

Ligands and Environments

2 bound ligands:
Ligand DMS 1 x DMS
Ligand SZY 1 x SZY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 42.737Å b: 62.308Å c: 147.251Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.214
Expression system: Escherichia coli

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