› 7got

X-ray diffraction
1.41Å resolution

PanDDA analysis group deposition -- Crystal Structure of Enterovirus D68 3C Protease in complex with Z26781952

Released:
DOI: 10.2210/pdb7got/pdb
PDB entry 7got coloured by chain, front view.
PDB entry 7got coloured by chain, side view.
PDB entry 7got coloured by chain, top view.
Source organism: enterovirus D68
Entry authors: Lithgo RM, Fairhead M, Koekemoer L, Aschenbrenner JC, Balcomb BH, Godoy AS, Marples PG, Ni X, Tomlinson CWE, Thompson W, Wild C, Fearon D, Walsh MA, von Delft F

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236123 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease 3C Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.13 KDa
Source organism: enterovirus D68
Expression system: Escherichia coli
UniProt:
  • Canonical: Q68T42 (Residues: 1548-1729; Coverage: 8%)
Sequence domains: 3C cysteine protease (picornain 3C)

Ligands and Environments

2 bound ligands:
Ligand W0A 1 x W0A
Ligand DMS 1 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 42.749Å b: 62.573Å c: 147.374Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.21
Expression system: Escherichia coli

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