› 7jh3

X-ray diffraction
2.68Å resolution

Crystal structure of 4-aminobutyrate aminotransferase PuuE from Escherichia coli in complex with PLP

Released:
DOI: 10.2210/pdb7jh3/pdb
PDB entry 7jh3 coloured by chain, front view.
PDB entry 7jh3 coloured by chain, side view.
PDB entry 7jh3 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Valleau D, Evdokimova E, Stogios PJ, Di Leo R, Savchenko A, Joachimiak A, Satchell KJF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156187 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-aminobutyrate aminotransferase PuuE Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 421 amino acids
Theoretical weight: 45 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50457 (Residues: 1-421; Coverage: 100%)
Gene names: JW1295, b1302, goaG, puuE
Sequence domains: Aminotransferase class-III

Ligands and Environments

1 bound ligand:
Ligand PEG 2 x PEG
1 modified residue:
Ligand LLP 6 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2
Unit cell:
a: 198.641Å b: 78.839Å c: 164.976Å
α: 90° β: 112.823° γ: 90°
R-values:
R R work R free
0.179 0.178 0.211
Expression system: Escherichia coli BL21(DE3)

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