7jhh

Electron Microscopy
3.92Å resolution

Cryo-EM structure of ATP-bound fully inactive AMPK in complex with Fab and nanobody

Released:

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein
ATP + [tau protein] = ADP + [tau protein] phosphate
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] = ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-208092 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (8 distinct):
5'-AMP-activated protein kinase catalytic subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 484 amino acids
Theoretical weight: 56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q13131 (Residues: 22-559; Coverage: 87%)
Gene names: AMPK1, PRKAA1
Sequence domains:
5'-AMP-activated protein kinase subunit beta-2 Chain: B
Molecule details ›
Chain: B
Length: 198 amino acids
Theoretical weight: 22.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: O43741 (Residues: 75-272; Coverage: 73%)
Gene name: PRKAB2
Sequence domains:
5'-AMP-activated protein kinase subunit gamma-1 Chain: G
Molecule details ›
Chain: G
Length: 306 amino acids
Theoretical weight: 34.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P54619 (Residues: 24-327; Coverage: 92%)
Gene name: PRKAG1
Sequence domains: CBS domain
Maltose/maltodextrin-binding periplasmic protein Chain: M
Molecule details ›
Chain: M
Length: 373 amino acids
Theoretical weight: 40.83 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0AEX9 (Residues: 25-392; Coverage: 99%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.21 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12
Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 237 amino acids
Theoretical weight: 25.48 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12
Nanobody Chain: N
Molecule details ›
Chain: N
Length: 159 amino acids
Theoretical weight: 17.41 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.92Å
Relevant EMDB volumes: EMD-22337
Expression system: Escherichia coli K-12