› 7jhj

Electron Microscopy
3.2Å resolution

Structure of the Epstein-Barr virus GPCR BILF1 in complex with human Gi

Released:
DOI: 10.2210/pdb7jhj/pdb
PDB entry 7jhj coloured by chain, front view.
PDB entry 7jhj coloured by chain, side view.
PDB entry 7jhj coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the constitutive activity and immunomodulatory properties of the Epstein-Barr virus-encoded G protein-coupled receptor BILF1.
Tsutsumi N, Qu Q, Mavri M, Baggesen MS, Maeda S, Waghray D, Berg C, Kobilka BK, Rosenkilde MM, Skiniotis G, Garcia KC
Immunity 54 1405-1416.e7 (2021)
PMID: 34216564
Related structures: EMD-22338

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-208889 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 40.28 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63096 (Residues: 2-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 67 amino acids
Theoretical weight: 7.43 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 2-68; Coverage: 94%)
Gene name: GNG2
Sequence domains: GGL domain
Antibody fragment scFv16 Chain: D
Molecule details ›
Chain: D
Length: 256 amino acids
Theoretical weight: 27.34 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
G-protein coupled receptor BILF1 Chain: R
Molecule details ›
Chain: R
Length: 321 amino acids
Theoretical weight: 35.49 KDa
Source organism: human gammaherpesvirus 4
Expression system: Homo sapiens
UniProt:
  • Canonical: P03208 (Residues: 1-312; Coverage: 100%)
Gene name: BILF1

Ligands and Environments

1 bound ligand:
Ligand Y01 1 x Y01
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-22338
Expression systems:
  • Trichoplusia ni
  • Homo sapiens

Quick links

Citations

3 review citations

Viral G Protein-Coupled Receptors Encoded by β- and γ-Herpesviruses.
Rosenkilde et al. (2022)
2 more

2 mentions without citation

Structural basis for the constitutive activity and immunomodulatory properties of the Epstein-Barr virus-encoded G protein-coupled receptor BILF1.
Tsutsumi et al. (2021)
1 more