› 7jig

X-ray diffraction
2.32Å resolution

HRAS A59T GppNHp crystal 2

Released:
DOI: 10.2210/pdb7jig/pdb
Model geometry
Fit model/data
PDB entry 7jig coloured by chain, front view.
PDB entry 7jig coloured by chain, side view.
PDB entry 7jig coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Regulation of GTPase function by autophosphorylation.
Johnson CW, Seo HS, Terrell EM, Yang MH, KleinJan F, Gebregiworgis T, Gasmi-Seabrook GMC, Geffken EA, Lakhani J, Song K, Bashyal P, Popow O, Paulo JA, Liu A, Mattos C, Marshall CB, Ikura M, Morrison DK, Dhe-Paganon S, Haigis KM
Mol Cell 82 950-968.e14 (2022)
PMID: 35202574

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli #1/H766
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family

Ligands and Environments

2 bound ligands:
Ligand GNP 1 x GNP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P3221
Unit cell:
a: 38.964Å b: 38.964Å c: 159.048Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.177 0.264
Expression system: Escherichia coli #1/H766

Quick links

Citations

2 review citations

The Predictive and Prognostic Role of RAS-RAF-MEK-ERK Pathway Alterations in Breast Cancer: Revision of the Literature and Comparison with the Analysis of Cancer Genomic Datasets.
Rocca et al. (2022)
1 more

1 mention without citation

Regulation of GTPase function by autophosphorylation.
Johnson et al. (2022)