7jpn

Electron Microscopy
3.24Å resolution

Cryo-EM structure of Arpin-bound Arp2/3 complex

Released:
Source organisms:
Related structures: EMD-22416

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-108368 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Actin-related protein 3 Chain: A
Molecule details ›
Chain: A
Length: 413 amino acids
Theoretical weight: 46.91 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61157 (Residues: 3-415; Coverage: 99%)
Gene names: ACTR3, ARP3
Sequence domains: Actin
Actin-related protein 2 Chain: B
Molecule details ›
Chain: B
Length: 377 amino acids
Theoretical weight: 42.95 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: A7MB62 (Residues: 4-380; Coverage: 96%)
Gene names: ACTR2, ARP2
Sequence domains: Actin
Actin-related protein 2/3 complex subunit 1B Chain: C
Molecule details ›
Chain: C
Length: 372 amino acids
Theoretical weight: 41.03 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q58CQ2 (Residues: 1-372; Coverage: 100%)
Gene name: ARPC1B
Sequence domains: WD domain, G-beta repeat
Actin-related protein 2/3 complex subunit 2 Chain: D
Molecule details ›
Chain: D
Length: 300 amino acids
Theoretical weight: 34.4 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3MHR7 (Residues: 1-300; Coverage: 100%)
Gene name: ARPC2
Sequence domains: Arp2/3 complex, 34 kD subunit p34-Arc
Actin-related protein 2/3 complex subunit 3 Chain: E
Molecule details ›
Chain: E
Length: 178 amino acids
Theoretical weight: 20.57 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T035 (Residues: 1-178; Coverage: 100%)
Gene name: ARPC3
Sequence domains: ARP2/3 complex ARPC3 (21 kDa) subunit
Actin-related protein 2/3 complex subunit 4 Chain: F
Molecule details ›
Chain: F
Length: 168 amino acids
Theoretical weight: 19.7 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q148J6 (Residues: 1-168; Coverage: 100%)
Gene name: ARPC4
Sequence domains: ARP2/3 complex 20 kDa subunit (ARPC4)
Actin-related protein 2/3 complex subunit 5 Chain: G
Molecule details ›
Chain: G
Length: 143 amino acids
Theoretical weight: 15.47 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: G3MXC8 (Residues: 9-48; Coverage: 27%)
Gene name: ARPC5
Arpin Chain: H
Molecule details ›
Chain: H
Length: 34 amino acids
Theoretical weight: 3.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z6K5 (Residues: 193-226; Coverage: 15%)
Gene names: ARPIN, C15orf38

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.24Å
Relevant EMDB volumes: EMD-22416
Expression system: Escherichia coli