› 7jqg

X-ray diffraction
2.15Å resolution

Crystal structure of human PPARgamma ligand binding domain Y473E mutant in complex with GW1929

Released:
DOI: 10.2210/pdb7jqg/pdb
PDB entry 7jqg coloured by chain, front view.
PDB entry 7jqg coloured by chain, side view.
PDB entry 7jqg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural mechanism underlying ligand binding and activation of PPARγ.
Shang J, Kojetin DJ
Structure 29 940-950.e4 (2021)
PMID: 33713599

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153368 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisome proliferator-activated receptor gamma Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 31.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37231 (Residues: 231-505; Coverage: 55%)
Gene names: NR1C3, PPARG
Sequence domains: Ligand-binding domain of nuclear hormone receptor

Ligands and Environments

1 bound ligand:
Ligand EDK 2 x EDK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 92.699Å b: 61.625Å c: 118.66Å
α: 90° β: 102.44° γ: 90°
R-values:
R R work R free
0.228 0.225 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Peroxisome Proliferator-Activated Receptor α in Lipoprotein Metabolism and Atherosclerotic Cardiovascular Disease.
Fuior et al. (2023)
1 more

1 mention without citation

Structural mechanism underlying ligand binding and activation of PPARγ.
Shang et al. (2021)