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ArfB can displace mRNA to rescue stalled ribosomes.

<div class='caption-title'>Catalytic activity of ArfB on 70S ribosomes with mRNAs of different lengths and sequences.</div>
<div class='caption-title'>Cryo-EM structures of the 70S complex formed with +2 mRNA and ArfB.</div>
<div class='caption-title'>Cryo-EM structures of the 70S complex formed with +9 mRNA and ArfB.</div>
Carbone CE, Demo G, Madireddy R, Svidritskiy E, Korostelev AA
OpenAccess logo Nat Commun 11 5552 (2020)
Related entries: 7jss, 7jsw, 7jt1, 7jt2, 7jt3

Cited: 7 times
EuropePMC logo PMID: 33144582

Abstract

Ribosomes stalled during translation must be rescued to replenish the pool of translation-competent ribosomal subunits. Bacterial alternative rescue factor B (ArfB) releases nascent peptides from ribosomes stalled on mRNAs truncated at the A site, allowing ribosome recycling. Prior structural work revealed that ArfB recognizes such ribosomes by inserting its C-terminal α-helix into the vacant mRNA tunnel. In this work, we report that ArfB can efficiently recognize a wider range of mRNA substrates, including longer mRNAs that extend beyond the A-site codon. Single-particle cryo-EM unveils that ArfB employs two modes of function depending on the mRNA length. ArfB acts as a monomer to accommodate a shorter mRNA in the ribosomal A site. By contrast, longer mRNAs are displaced from the mRNA tunnel by more than 20 Å and are stabilized in the intersubunit space by dimeric ArfB. Uncovering distinct modes of ArfB function resolves conflicting biochemical and structural studies, and may lead to re-examination of other ribosome rescue pathways, whose functions depend on mRNA lengths.

Reviews - 7jsz mentioned but not cited (1)

  1. Ribosome Rescue Pathways in Bacteria. Müller C, Crowe-McAuliffe C, Wilson DN. Front Microbiol 12 652980 (2021)


Reviews citing this publication (2)

  1. Bacterial Ribosome Rescue Systems. Kurita D, Himeno H. Microorganisms 10 372 (2022)
  2. Diversity and Similarity of Termination and Ribosome Rescue in Bacterial, Mitochondrial, and Cytoplasmic Translation. Korostelev AA. Biochemistry (Mosc) 86 1107-1121 (2021)

Articles citing this publication (4)

  1. Structures of tmRNA and SmpB as they transit through the ribosome. Guyomar C, D'Urso G, Chat S, Giudice E, Gillet R. Nat Commun 12 4909 (2021)
  2. Nonstop mRNAs generate a ground state of mitochondrial gene expression noise. Ng KY, Lutfullahoglu Bal G, Richter U, Safronov O, Paulin L, Dunn CD, Paavilainen VO, Richer J, Newman WG, Taylor RW, Battersby BJ. Sci Adv 8 eabq5234 (2022)
  3. Quality Control Mechanisms in Bacterial Translation. Zarechenskaia AS, Sergiev PV, Osterman IA. Acta Naturae 13 32-44 (2021)
  4. Sequential rescue and repair of stalled and damaged ribosome by bacterial PrfH and RtcB. Tian Y, Zeng F, Raybarman A, Fatma S, Carruthers A, Li Q, Huang RH. Proc Natl Acad Sci U S A 119 e2202464119 (2022)


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