› 7jth

Electron Microscopy
4Å resolution

Cryo-EM structure of unliganded octameric prenyltransferase domain of Phomopsis amygdali fusicoccadiene synthase

Released:
DOI: 10.2210/pdb7jth/pdb
PDB entry 7jth coloured by chain, front view.
PDB entry 7jth coloured by chain, side view.
PDB entry 7jth coloured by chain, top view.
Source organism: Diaporthe amygdali
Primary publication:
Structural insight on assembly-line catalysis in terpene biosynthesis.
Faylo JL, van Eeuwen T, Kim HJ, Gorbea Colón JJ, Garcia BA, Murakami K, Christianson DW
OpenAccess logo Nat Commun 12 3487 (2021)
PMID: 34108468
Related structures: EMD-22473

Function and Biology Details

Reactions catalysed: 
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate
Geranylgeranyl diphosphate = fusicocca-2,10(14)-diene + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fusicoccadiene synthase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 739 amino acids
Theoretical weight: 83.88 KDa
Source organism: Diaporthe amygdali
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A2PZA5 (Residues: 1-719; Coverage: 100%)
Gene names: PaFS, orf1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-22473
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 citation in other articles

Discovery of non-squalene triterpenes.
Tao et al. (2022)
8 more

3 mentions without citation

Assembly-Line Catalysis in Bifunctional Terpene Synthases.
Faylo et al. (2021)
2 more