7jtr

X-ray diffraction
2.5Å resolution

Complex of maltose-binding protein (MBP) with single-chain Fv (scFv)

Released:
Model geometry
Fit model/data
Source organisms:
Primary publication:
A useful epitope tag derived from maltose binding protein.
Protein Sci 30 1235-1246 (2021)
PMID: 33896065

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-225142 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Maltose/maltodextrin-binding periplasmic protein Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 369 amino acids
Theoretical weight: 40.65 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-395; Coverage: 100%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
single-chain Fv antibody fragment (scFv) Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 251 amino acids
Theoretical weight: 27.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 95.2Å b: 91.7Å c: 174.63Å
α: 90° β: 91.44° γ: 90°
R-values:
R R work R free
0.201 0.2 0.237
Expression system: Escherichia coli