› 7ju3

X-ray diffraction
2.7Å resolution

MtrR bound to the mtrCDE operator from Neisseria gonorrhoeae

Released:
DOI: 10.2210/pdb7ju3/pdb
PDB entry 7ju3 coloured by chain, front view.
PDB entry 7ju3 coloured by chain, side view.
PDB entry 7ju3 coloured by chain, top view.
Source organism: Neisseria gonorrhoeae
Primary publication:
Structures of Neisseria gonorrhoeae MtrR-operator complexes reveal molecular mechanisms of DNA recognition and antibiotic resistance-conferring clinical mutations.
Beggs GA, Ayala JC, Kavanaugh LG, Read TD, Hooks GM, Schumacher MA, Shafer WM, Brennan RG
OpenAccess logo Nucleic Acids Res 49 4155-4170 (2021)
PMID: 33784401

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121408 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH-type transcriptional regulator MtrR Chains: A, B
Molecule details ›
Chains: A, B
Length: 213 amino acids
Theoretical weight: 24.5 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli
UniProt:
  • Canonical: P39897 (Residues: 1-210; Coverage: 100%)
Gene name: mtrR
Sequence domains:
DNA (5'-D(*TP*AP*TP*CP*CP*GP*TP*GP*CP*AP*AP*TP*CP*GP*TP*GP*TP*AP*TP*GP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli
DNA (5'-D(*AP*CP*AP*TP*AP*CP*AP*CP*GP*AP*TP*TP*GP*CP*AP*CP*GP*GP*AP*TP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 153.273Å b: 59.42Å c: 69.508Å
α: 90° β: 114.31° γ: 90°
R-values:
R R work R free
0.233 0.228 0.282
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Transcriptional regulation of the mtrCDE efflux pump operon: importance for Neisseria gonorrhoeae antimicrobial resistance.
Ayala et al. (2022)
6 other articles

1 mention without citation

Structures of Neisseria gonorrhoeae MtrR-operator complexes reveal molecular mechanisms of DNA recognition and antibiotic resistance-conferring clinical mutations.
Beggs et al. (2021)