› 7ju4

Electron Microscopy
3.4Å resolution

Radial spoke 2 stalk, IDAc, and N-DRC attached with doublet microtubule

Released:
DOI: 10.2210/pdb7ju4/pdb
PDB entry 7ju4 coloured by chain, front view.
PDB entry 7ju4 coloured by chain, side view.
PDB entry 7ju4 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Structures of radial spokes and associated complexes important for ciliary motility.
Gui M, Ma M, Sze-Tu E, Wang X, Koh F, Zhong ED, Berger B, Davis JH, Dutcher SK, Zhang R, Brown A
OpenAccess logo Nat Struct Mol Biol 28 29-37 (2021)
PMID: 33318703
Related structures: EMD-22481

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 61-mer (preferred)
PDBe Complex ID:
PDB-CPX-100802 (preferred)
Entry contents:
19 distinct polypeptide molecules
Macromolecules (19 distinct):
Dynein regulatory complex subunit 4 Chains: 0, 4
Molecule details ›
Chains: 0, 4
Length: 471 amino acids
Theoretical weight: 55.21 KDa
Source organism: Chlamydomonas reinhardtii
Dynein regulatory complex protein 1 Chain: 1
Molecule details ›
Chain: 1
Length: 698 amino acids
Theoretical weight: 79.45 KDa
Source organism: Chlamydomonas reinhardtii
Dynein regulatory complex subunit 2 Chain: 2
Unknown protein Chain: 3
Tubulin beta-1/beta-2 chain Chains: 6, 8, C, G, I, K, O, Q, S, W, Y, i, k, y
Tubulin alpha-1 chain Chains: 7, 9, D, H, J, L, P, R, T, X, Z, j, t, z
Flagellar-associated protein 59 Chain: A
Flagellar-associated protein 172 Chain: B
Radial spoke protein 3 Chains: E, F
EF-hand domain-containing protein Chains: M, N
RIIa domain-containing protein Chains: U, V
Dynein 8 kDa light chain, flagellar outer arm Chains: a, b, c, d, e, f, g, h, o, p, q, r
Flagellar associated protein Chain: l
MORN repeat-containing protein 3 Chain: m
Radial spike protein 8 Chain: n
IQ and ubiquitin-like domain-containing protein Chain: s
Actin Chain: u
28 kDa inner dynein arm light chain, axonemal Chains: v, w
Cilia- and flagella-associated protein 91 Chain: x

Ligands and Environments

4 bound ligands:
Ligand GDP 14 x GDP
Ligand GTP 14 x GTP
Ligand MG 14 x MG
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-22481

Quick links

Citations

9 review citations

Uncovering structural ensembles from single-particle cryo-EM data using cryoDRGN.
Kinman et al. (2023)
8 more

6 mentions without citation

Structures of radial spokes and associated complexes important for ciliary motility.
Gui et al. (2021)
5 more