› 7jzw

Electron Microscopy
3.2Å resolution

Cryo-EM structure of CRISPR-Cas surveillance complex with AcrIF4

Released:
DOI: 10.2210/pdb7jzw/pdb
PDB entry 7jzw coloured by chain, front view.
PDB entry 7jzw coloured by chain, side view.
PDB entry 7jzw coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for inhibition of the type I-F CRISPR-Cas surveillance complex by AcrIF4, AcrIF7 and AcrIF14.
Gabel C, Li Z, Zhang H, Chang L
OpenAccess logo Nucleic Acids Res 49 584-594 (2021)
PMID: 33332569
Related structures: EMD-22582

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-125828 (preferred)
Entry contents:
5 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (6 distinct):
CRISPR-associated protein Csy1 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 49.14 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02ML9 (Residues: 1-434; Coverage: 100%)
Gene names: PA14_33330, csy1
Sequence domains: CRISPR-associated protein (Cas_Csy1)
CRISPR-associated endonuclease Cas6/Csy4 Chain: C
Molecule details ›
Chain: C
Length: 187 amino acids
Theoretical weight: 21.43 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM2 (Residues: 1-187; Coverage: 100%)
Gene names: PA14_33300, cas6f, csy4
Sequence domains: CRISPR-associated protein (Cas_Csy4)
CRISPR-associated protein Csy2 Chain: B
Molecule details ›
Chain: B
Length: 329 amino acids
Theoretical weight: 36.45 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM0 (Residues: 1-327; Coverage: 100%)
Gene names: PA14_33320, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
CRISPR-associated protein Csy3 Chains: D, E, F, G, H, I
Molecule details ›
Chains: D, E, F, G, H, I
Length: 344 amino acids
Theoretical weight: 37.78 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM1 (Residues: 1-342; Coverage: 100%)
Gene names: PA14_33310, csy1-3, csy3
Sequence domains: CRISPR-associated protein (Cas_Csy3)
Type I-F anti-CRISPR protein Chain: J
Molecule details ›
Chain: J
Length: 100 amino acids
Theoretical weight: 11.09 KDa
Source organism: Pseudomonas phage sp.
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: L7P7U3 (Residues: 1-100; Coverage: 100%)
Gene name: JBD24_036
CRISPR repeat sequence Chain: M
Molecule details ›
Chain: M
Length: 61 nucleotides
Theoretical weight: 19.54 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-22582
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins.
Jia et al. (2021)
1 more

4 mentions without citation

Structural insights into the inactivation of the type I-F CRISPR-Cas system by anti-CRISPR proteins.
Yang et al. (2021)
3 more