› 7k17

X-ray diffraction
4.3Å resolution

Re-refined crystal structure of DNA-dependent protein kinase catalytic subunit complexed with Ku80 C-terminal helix

Released:
DOI: 10.2210/pdb7k17/pdb
PDB entry 7k17 coloured by chain, front view.
PDB entry 7k17 coloured by chain, side view.
PDB entry 7k17 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of an activated DNA-PK and its implications for NHEJ.
Chen X, Xu X, Chen Y, Cheung JC, Wang H, Jiang J, de Val N, Fox T, Gellert M, Yang W
Mol Cell 81 801-810.e3 (2021)
PMID: 33385326

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146434 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-dependent protein kinase catalytic subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 3986 amino acids
Theoretical weight: 452.52 KDa
Source organism: Homo sapiens
X-ray repair cross-complementing protein 5 Chains: C, D
Molecule details ›
Chains: C, D
Length: 192 amino acids
Theoretical weight: 21.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13010 (Residues: 541-732; Coverage: 26%)
Gene names: G22P2, XRCC5
Sequence domains: Ku C terminal domain like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29, ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 169.12Å b: 132.64Å c: 296.59Å
α: 90° β: 105.53° γ: 90°
R-values:
R R work R free
0.287 0.286 0.335
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Function and Molecular Mechanism of the DNA Damage Response in Immunity and Cancer Immunotherapy.
Ye et al. (2021)
17 more

1 mention without citation

Structure of an activated DNA-PK and its implications for NHEJ.
Chen et al. (2021)