› 7k1d

X-ray diffraction
3Å resolution

Crystal structure of human insulin degrading enzyme (IDE) in complex with compound BDM_77291

Released:
DOI: 10.2210/pdb7k1d/pdb
PDB entry 7k1d coloured by chain, front view.
PDB entry 7k1d coloured by chain, side view.
PDB entry 7k1d coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Liang WG, Deprez R, Bosc D, Tang W

Function and Biology Details

Reaction catalysed: 
Degradation of insulin, glucagon and other polypeptides. No action on proteins.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147045 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Insulin-degrading enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 990 amino acids
Theoretical weight: 114.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P14735 (Residues: 42-1019; Coverage: 96%)
Gene name: IDE
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand VQD 2 x VQD
Ligand DIO 6 x DIO
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 264.313Å b: 264.313Å c: 90.468Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.156 0.192
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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