› 7k21

Electron Microscopy
3Å resolution

Cryo-EM structure of pyrene-labeled ADP-Pi-actin filaments

Released:
DOI: 10.2210/pdb7k21/pdb
PDB entry 7k21 coloured by chain, front view.
PDB entry 7k21 coloured by chain, side view.
PDB entry 7k21 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments.
Chou SZ, Pollard TD
OpenAccess logo Nat Commun 11 5897 (2020)
PMID: 33214556
Related structures: EMD-22639

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, alpha skeletal muscle Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P68139 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand ADP 4 x ADP
Ligand PO4 4 x PO4
Ligand 1T4 4 x 1T4
1 modified residue:
Ligand HIC 4 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-22639

Quick links

Citations

2 review citations

Deciphering the molecular mechanisms of actin cytoskeleton regulation in cell migration using cryo-EM.
Fäßler et al. (2023)
1 more

2 mentions without citation

High-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism.
Pospich et al. (2021)
1 more