7k52

Electron Microscopy
3.4Å resolution

Near post-translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure III)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 59-mer (preferred)
PDBe Complex ID:
PDB-CPX-241233 (preferred)
Entry contents:
53 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (59 distinct):
Large ribosomal subunit protein uL2 Chain: b
Molecule details ›
Chain: b
Length: 271 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P60422 (Residues: 2-272; Coverage: 99%)
Gene names: JW3279, b3317, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: c
Molecule details ›
Chain: c
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: d
Large ribosomal subunit protein uL5 Chain: e
Large ribosomal subunit protein uL6 Chain: f
Large ribosomal subunit protein bL9 Chain: g
Large ribosomal subunit protein uL10 Chain: h
Large ribosomal subunit protein uL11 Chain: i
Large ribosomal subunit protein uL13 Chain: j
Large ribosomal subunit protein uL14 Chain: k
Large ribosomal subunit protein uL15 Chain: l
Large ribosomal subunit protein uL16 Chain: m
Large ribosomal subunit protein bL17 Chain: n
Large ribosomal subunit protein uL18 Chain: o
Large ribosomal subunit protein bL19 Chain: p
Large ribosomal subunit protein bL20 Chain: q
Large ribosomal subunit protein bL21 Chain: r
Large ribosomal subunit protein uL22 Chain: s
Large ribosomal subunit protein uL23 Chain: t
Large ribosomal subunit protein uL24 Chain: u
Large ribosomal subunit protein bL25 Chain: v
Large ribosomal subunit protein bL27 Chain: w
Large ribosomal subunit protein bL28 Chain: x
Large ribosomal subunit protein uL29 Chain: y
Large ribosomal subunit protein uL30 Chain: z
Large ribosomal subunit protein bL31 Chain: A
Large ribosomal subunit protein bL32 Chain: B
Large ribosomal subunit protein bL33 Chain: C
Large ribosomal subunit protein bL34 Chain: D
Large ribosomal subunit protein bL35 Chain: E
Large ribosomal subunit protein bL36A Chain: F
Small ribosomal subunit protein uS2 Chain: G
Small ribosomal subunit protein uS3 Chain: H
Small ribosomal subunit protein uS4 Chain: I
Small ribosomal subunit protein uS5 Chain: J
Small ribosomal subunit protein bS6, non-modified isoform Chain: K
Small ribosomal subunit protein uS7 Chain: L
Small ribosomal subunit protein uS8 Chain: M
Small ribosomal subunit protein uS9 Chain: N
Small ribosomal subunit protein uS10 Chain: O
Small ribosomal subunit protein uS11 Chain: P
Small ribosomal subunit protein uS12 Chain: Q
Small ribosomal subunit protein uS13 Chain: R
Small ribosomal subunit protein uS14 Chain: S
Small ribosomal subunit protein uS15 Chain: T
Small ribosomal subunit protein bS16 Chain: U
Small ribosomal subunit protein uS17 Chain: V
Small ribosomal subunit protein bS18 Chain: W
Small ribosomal subunit protein uS19 Chain: X
Small ribosomal subunit protein bS20 Chain: Y
Small ribosomal subunit protein bS21 Chain: Z
Large ribosomal subunit protein uL1 Chain: a
Elongation factor G Chain: 8
16S ribosomal RNA Chain: 3
23S ribosomal RNA Chain: 1
5S ribosomal RNA Chain: 2
tRNAPro Chain: 5
tRNAfMet Chain: 6
Messenger RNA Chain: 4

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-22671
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'