› 7k9e

X-ray diffraction
2.1Å resolution

Crystal structure of Bacillus halodurans OapB in complex with its OLE RNA target (crystal form II)

Released:
DOI: 10.2210/pdb7k9e/pdb
PDB entry 7k9e coloured by chain, front view.
PDB entry 7k9e coloured by chain, side view.
PDB entry 7k9e coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Primary publication:
Structure of a bacterial OapB protein with its OLE RNA target gives insights into the architecture of the OLE ribonucleoprotein complex.
Yang Y, Harris KA, Widner DL, Breaker RR
OpenAccess logo Proc Natl Acad Sci U S A 118 (2021)
PMID: 33619097

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191880 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribosomal protein L14E/L6E/L27E Chains: A, C
Molecule details ›
Chains: A, C
Length: 98 amino acids
Theoretical weight: 11.38 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KGD7 (Residues: 5-102; Coverage: 96%)
Gene name: BH0157
OLE* RNA Chains: B, D
Molecule details ›
Chains: B, D
Length: 60 nucleotides
Theoretical weight: 19.47 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GTP 2 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P65
Unit cell:
a: 71.99Å b: 71.99Å c: 223.905Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.172 0.204
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 citations in other articles

Ornate, large, extremophilic (OLE) RNA forms a kink turn necessary for OapC protein recognition and RNA function.
Lyon et al. (2022)