› 7kat

Electron Microscopy
4.4Å resolution

Cryo-EM structure of the Sec complex from S. cerevisiae, Sec61 pore ring and Sec63 FN3 double mutant, class without Sec62

Released:
DOI: 10.2210/pdb7kat/pdb
PDB entry 7kat coloured by chain, front view.
PDB entry 7kat coloured by chain, side view.
PDB entry 7kat coloured by chain, top view.
Source organism: Saccharomyces cerevisiae BY4741
Primary publication:
Stepwise gating of the Sec61 protein-conducting channel by Sec63 and Sec62.
Itskanov S, Kuo KM, Gumbart JC, Park E
OpenAccess logo Nat Struct Mol Biol 28 162-172 (2021)
PMID: 33398175
Related structures: EMD-22783

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-147139 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Protein transport protein SEC61 Chain: A
Molecule details ›
Chain: A
Length: 480 amino acids
Theoretical weight: 52.94 KDa
Source organism: Saccharomyces cerevisiae BY4741
UniProt:
  • Canonical: P32915 (Residues: 1-480; Coverage: 100%)
Gene names: L3502.5, SEC61, YLR378C
Sequence domains:
Protein transport protein SSS1 Chain: C
Molecule details ›
Chain: C
Length: 80 amino acids
Theoretical weight: 8.96 KDa
Source organism: Saccharomyces cerevisiae BY4741
UniProt:
  • Canonical: P35179 (Residues: 1-80; Coverage: 100%)
Gene names: D4475, SSS1, YDR086C
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
Protein transport protein SBH1 Chain: B
Protein translocation protein SEC63 Chain: D
Translocation protein SEC66 Chain: E
Translocation protein SEC72 Chain: F

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-22783
Expression system: Saccharomyces cerevisiae BY4741

Quick links

Citations

11 review citations

The mechanisms of integral membrane protein biogenesis.
Hegde et al. (2022)
10 more

1 mention without citation

Stepwise gating of the Sec61 protein-conducting channel by Sec63 and Sec62.
Itskanov et al. (2021)